Besides the biological processes, the CALAGUA group also studies the microbiology of wastewater treatment systems by applying basic techniques such as phase contrast observation and differential stains, as well as molecular techniques like FISH (fluorescence in situ hibridization) or qPCR (quantitative polymerase chain reaction).

We also work on biological systems from the microbial diversity perspective applying NGS (next-generation sequencing) techniques.

CALAGUA continues to work on developing methods of determining WWTP simulator kinetic and stoichiometric parameters by off-line tests, and has managed to create automatic tests to calibrate these models with the BioCalibra device.